We have developed modules to capture the classifications of ‘hot’, ‘altered’ and ‘cold’ immune-tumor environments. In partnership with Immunaccel we have access to proprietary, well characterized, HLA-matched CTL and Tumor cell lines that includes a Keytruda resistant TIL and Tumor and Keytruda sensitive TIL and Tumor.
We have conducted extensive screening studies in evaluating lymphocyte migration and infiltration in response to a drug candidate. Our typical readouts include flow cytometry changes of checkpoint molecules on immune cells, multiplex cytokine readouts and immunohistochemical techniques. Example studies include: