Overview

IMMUNE 3D ® is a unique and proprietary platform customized to capture the human cellular microenvironment best suited to screen or discover drug candidates that impact. T cell migration and their effector functions. T cell migration is governed by mechanisms that regulate the activation state of the cell and for environment-specific cues. A number of factors including chemokines, stromal factors and matrix metalloproteinases have been shown to influence the infiltration of T cells in the tumor microenvironment (TME). The migration and infiltration of T cells is critical for effective antitumor immune surveillance. Studies have shown that T cells accumulate in the stroma and the density of stroma extracellular matrix plays a role in controlling migration of lymphocytes

 

Importance of Lymphocyte Migration

T cell migration is important for mounting an effective immune responses. It permits the T cell to recognize antigen presented in the context of MHC by an antigen presenting cells and then traffic and interact with other cells. Studying the interactions of immune cells and diseased cells or tissues can lead to the a better understanding of migration of immune cells 

 

Patient-derived cells or tumors are co-cultured with extracellular components such as fibroblasts and collagen. Lymphocytes (autologous or allogenic) are added to the platform and migration is observed at various time points enabling the study of important cell-cell interactions

Study the contribution of immunosuppressive cells such as Regulatory T cell (Tregs), Myeloid Derived Suppressor Cells (MDSCs) and Macrophages towards immune dysfunction

Study receptor-ligand interactions of immune cells under disease based conditions

Cancers often rapidly mutate and mutations in the genome can cause these malignant cells to express mutant proteins that are tumor specific and not expressed on normal cells, referred to as neoantigens. IMMUNE 3D® can be used to uniquely expand tumor-specific T cell populations that respond to novel neoantigens.

Chemokines are known for their ability to modulate lymphocyte recruitment and migration across diseases.

The disease environments are composed of surrounding stroma, which includes fibroblasts, endothelial cells, and immune cells, as well as the extracellular matrix. These cells contribute towards immune profile changes. Studying these interactions can lead to better therapies or biomarkers

Study factors such as role of immune cell migration such as Tregs, skin gamma-delta (γδ) T cells and their role in oncology and other diseases, Innate Lymphoid Cells (ILCs) and microbial interactions and the evolution of the immune response to aged matrix

Study immunometabolism factors such as lactic acid and ROS can be measured in the presence and absence of treatment

Advantages

IMMUNE 3D®’s design relies on natural physiological relevance and lymphocyte migration compared to other platforms that utilize artifical inserts to provide the added layer for evaluating lymphocyte migration.

  • Uniformity across all wells compared to spheroids
  • Cost-effective and simple design
  • Scalable to 96 wells
  • Adaptable to any fresh tumor tissues to evaluate migration and infiltration of lymphocytes
  • Customizable to capture disease environments and immune cells
  • Patented system and extensive know-how

Broad Spectrum Applications